Protein_Domain

Part:BBa_K1390004:Design

Designed by: Stefan Dübel   Group: iGEM14_Braunschweig   (2014-07-03)


mmoX


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1154
    Illegal BamHI site found at 1188
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 163
    Illegal BsaI.rc site found at 301
    Illegal BsaI.rc site found at 718


Design Notes

the brick is constructed in the RCF10 standard


Source

Amplified from the genomic DNA of Methylococcus capsulatus bath without any PstI restriction site

References

Other parts for the complete mmo enzyme:
mmoB: BBa_K1390001
mmoC: BBa_K1390002
mmoD: BBa_K1390003
mmoY: BBa_K1390005
mmoZ: BBa_K1390006